Microfluidics

  • Bubble dissolution
    • Bubble dissolution

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  • Bubbles and surfactants
    • Bubbles and surfactants

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  • Protein encapsulation
    • Protein encapsulation

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Our research in microfluidics aims at developing lab-on-a-chips, i.e. performing chemical or biological processing at small-scale. Our activity is organised around the miniaturization of physico-chemical unit operations, such as liquid/liquid separation by membrane pervaporation [1,6], continuous flow crystallisation [2,12,13] and bubble dissolution in bubbly flow regime [3,4,10]. Integrating one or several of these unit operations should lead to innovative and competing lab-on-a-chip devices for the fields of fine chemistry, pharmaceuticals and biotechnology.

Bubbles and droplets are elementary components in microfluidics and deserve a special attention as they are widely used in two-phase microfluidic applications. Therefore, modelling and predicting their dynamics in microchannels is of utmost importance. We thus investigate on the one hand the dynamics of confined (Taylor) bubbles, and especially the influence of buoyancy [7] and dewetting [8] on the lubrication film, developing in parallel an original method to measure its thickness [14]. On the other hand, we study the dynamics of unconfined bubbles in microchannels, paying a special attention to the inertial and capillary migration forces [9], as well as to the role of surfactants [11].

Note we have in parallel developed an droplet generator, referred to as the “Dropbox technology” and allowing to produce monodisperse droplets in dripping mode at high throughput [15]. This generator does not require surface treatment so any type of emulsification can be obtained with this device, such as oil in water, water in oil, bubbles or even partially miscible emulsions.

Applications to life sciences are also investigated such as cell/cell adhesion in micro channels [5], micro beads fabrication for protein purification, micro encapsulation of proteins or single-bacterium analysis and sorting [ongoing researches]

[15] Scheid B., Dewandre A. & Vitry Y., Droplet and/or bubble generator, PCT/EP2018/067960 (2019)
[14] Atasi O., Haut B., Dehaeck S., Dewandre A., Legendre D. & Scheid B., How to measure the thickness of a lubrication film in a pancake bubble with a single bright-field image?Applied Physics Letters 113, 173701 (Editor’s pick)
[13] Rimez B., Conté J., Norrant E., Cognet P., Gourdon C. & Scheid B., Continuous-Flow Tubular Crystallization to Discriminate between Two Competing Crystal Polymorphs. Part II: anti solvent crystallisationCrystal Growth & Design 18, 6440–6447
[12] Rimez B., Debuysschère R., Conté J., Norrant E., Cognet P., Gourdon C. & Scheid B., Continuous-Flow Tubular Crystallization to Discriminate between Two Competing Crystal Polymorphs. Part I: cooling crystallisationCrystal Growth & Design 18, 6431–6439
[11] Atasi O., Haut B., Pedrono A., Scheid B. & Legendre D., Influence of Soluble Surfactants and Deformation on the Dynamics of Centered Bubbles in Cylindrical MicrochannelsLangmuir 34, 10048-10062
[10] Rivero-Rodriguez J. & Scheid B., Mass transfer around flowing bubbles in cylindrical microchannels, Accepted to Journal of Fluid Mechanics
[9] Rivero-Rodriguez J. & Scheid B., Bubble dynamics in microchannels: inertial and capillary migration forcesJournal of Fluid Mechanics 842, 215-247 (2018)
[8] khodaparast S., Atasi O., Debris A., Scheid B. & Stone H.A., Dewetting of thin liquid films surrounding air bubbles in microchannelsLangmuir 34, 1363-1370 (2018)
[7] Atasi O., Khodaparast S., Scheid B. & Stone H.A., Effect of buoyancy on the motion of a long bubble in a horizontal tube, Physical Review Fluids 2, 094304 (2017)
[6] Ziemecka I., Haut B. & Scheid B., Continuous separation, with microfluidics, of the components of a ternary mixture: from vacuum to purge gas pervaporation, Microfluidics & Nanofluidics 21, 84 (2017)
[5] Petit A.-E., Demotte N., Scheid B., Wildmann C., Bigirimana R., Gordon-Alonso M., Carrasco J., Valitutti S., Godelaine D. & van der Bruggen P., A major secretory defect of tumour-infiltrating T lymphocytes due to galectin impairing LFA-1-mediated synapse completion, Nature Communications 712242 (2016)
[4] Mikaelian D., Haut B. & Scheid B., Bubbly flow and gas-liquid mass transfer in square and circular microchannels for stress-free and rigid interfaces: dissolution model, Microfluidics & Nanofluidics 19, 899-911 (2015)
[3] Mikaelian D., Haut B. & Scheid B., Bubbly flow and gas-liquid mass transfer in square and circular microchannels for stress-free and rigid interfaces: CFD analysis, Microfluidics & Nanofluidics 19, 523-545 (2015)
[2] Rimez B., Haut B. & Scheid B., Development of a continuous “self-seeding” microfluidic crystallization device for active pharmaceutical ingredients, BIWIC 2014 conference, Rouen (France) (2015)
[1] Ziemecka, I., Haut B. & Scheid B., Hydrogen peroxide concentration by pervaporation of a ternary liquid solution in microfluidicsLab on a chip 15, 504 (2015)